What is DNA “massaging”?
DNA “massage”, like a massage treatment, is a tool that helps the amateur bioinformatician handle and process DNA sequences.
There are many DNA sequencing tools and software on the market. Other professional teams employ bioinformatics experts that can do also script coding and handle many more complicated tasks professionally.
What most of us working with common genetics application do in everyday bases:
- Design probes
- Analyze Sanger sequences
- Find SNPs
- Compare different sequences
DNA sequences comes in different formats: FASTA files are most common
The main problem with FASTA format is that it contains line breaks. For BLAST and some primer design SW this does not matter but for some it does. It also makes it challenging to edit these sequences for communicating them in word processors or presentation software.
1 tgaatctgga gaaggtggca atgagctcat caagagtgtc ctccagtcca tattgagcta
61 tcctggctac tgagaataac ccttcaatgc attcatggag caactcctct tcttcagctt
121 gctcaaaaaa tgcagaaata gctgcgactg atgggccagc aatgcaagca aacatgtctc
181 tccctaaccg gcgatcaaaa tcacatagaa tgaaaggctg cacagttttg gatcggttca
241 tcaactcaat ccatctgctt gggttcatta ttactggcag accggattga ccaaaaaggg
301 tgattgcatt gtttgatatg gac
We recently created an internal offline xls file to do exactly as the online tool does and it also has some advantages over it. We can share this tool with you if you want.